Peaks Studio 11.5 full crack download unlimited
For new users looking to try PEAKS Studio, we always recommend testing your own data on the latest version. With a 15-day free trial, users can test the complete version of PEAKS Studio with all identification and quantitative features and tools.
PEAKS Studio 12 supports Windows operating systems. PEAKS Studio 12 is recommended to be installed on 64-bit Windows operating systems with Windows 10 or later. See full system requirements below.
PEAKS Studio 12 installer will take ~4 GB of disk space and PEAKS 12 program files will use ~7 GB of disk space.
It is recommended to allocate a large disk space for the location of running projects, and ensure there is space in the PEAKS Studio installation directory for configuring databases. Configuring protein databases and glycan databases may require up to double the file size of the configured .fasta file.
The installation link of the latest version of PEAKS Studio 12 can be requested from the form below.
1. Peptide/Protein Identification
De novo sequencing.
Database search with new deep learning enhancements to maximize peptide ID efficiency.
DIA workflow (spectral library search + directDB + de novo).
New: Direct database search using DIA data with higher sensitivity and accuracy (including DIA with short gradients).
Enhanced deep learning-based technology for predicting spectra, retention times, and collision cross-section values.
Post-translational modification (PTM) search with 500+ modifications.
Sequence variation and mutation search.
New: DeepNovo-based immunopeptidomics Peptidomics workflow: Integrate homology search with de novo to improve HLA peptidomics under FDR control.
New: PEAKS glycan module for highly sensitive and accurate glycoproteomics
2. Excellent label-free and label-based quantification
Label-free and label-based: TMT (MS2, MS3)/iTRAQ, SILAC, 18D label, ICAT, user-defined.
Quantitative results can be visualized using heatmaps, correlation curves, and extracted ion chromatograms (XICs).
3. Easy-to-use interface and excellent results visualization
Detailed and easy-to-use graphical user interface (GUI) for viewing, filtering, and verifying results.
Spectral library viewer to assess quality and verify libraries before use.
Statistical calculations presented in a visual way to assess the quality of raw data and/or results.
LC-MS/MS heatmaps provide a comprehensive display of peptide features, MS/MS spectrum acquisition, and identification positions relative to mass overcharge (m/z), retention time (RT), compensation voltage (CV), ion mobility (1/k0), and signal intensity.
4. Vendor neutral and fine-tuned to take advantage of the latest MS technology
New: PEAKS 11 now supports ZenoTOF.
Support for ion mobility spectroscopic proteomics (timsTOF Pro, FAIMS, HDMSe).
Algorithms are fine-tuned for each instrument and fragmentation type to ensure optimal accuracy and sensitivity.
Fully support DDA and DIA for identification and quantification.
Software Features
1. Feature-based identification workflow to increase sensitivity and maximize peptide identification efficiency.
Designed specifically for DDA technology to improve reproducibility.
Integrate database search and de novo sequencing to expand in-depth analysis.
Activate deep learning boosting in the PEAKS DDA workflow to maximize peptide identification efficiency.
2. Streamline workflow with direct database search DIA
DIA analysis is an attractive alternative to the DDA workflow. In the past, DIA methods relied on generating spectral libraries from DDA to identify and quantify peptides. PEAKS Studio 11 offers a unique DIA workflow that maximizes the identification rate of peptides by integrating three methods: library search, direct database search, and de novo sequencing.
Perform library searches against predefined spectral libraries. Peptide spectra without library matches can be searched directly in protein databases.
Directly search protein sequence databases using DIA data. Advanced machine learning algorithms can improve the accuracy and sensitivity of peptide identification. In this step of the pipeline, the PEAKS 11 DIA workflow now supports the identification of any PTM specified by the user. This will enable improved identification of modified peptides without having to enter them into a library.
Spectra that do not match in the database search are sequenced de novo.
Peptides identified from library searches and protein sequence database searches can be used for quantitative analysis.
Note: All 3 search methods, PEAKS library search, direct database search, and de novo sequencing are optional, and the steps of the workflow can be performed independently or in sequence.
3. PEAKS DeepNovo Peptidomics: Advanced Solution for Immunopeptidomics
This newly developed solution is a dedicated workflow for peptidomics data that combines database search, de novo sequencing, and mutant peptide identification.
4. Ion mobility enables quantification to minimize missing values and improve accuracy
5. PEAKS Glycan Module Plug-in enables scientists to determine glycan site positioning and glycan structure
The PEAKS Glycan Module is a comprehensive data analysis tool that provides a highly sensitive and accurate glycoproteomics software solution to advance our understanding of the glycoproteome. Learn more
A comprehensive understanding of glycosylation and glycoproteins
Advanced glycoproteomics for deep glycan profiling
Innovative software tools for glycan and glycopeptide quantification
6. Quality Control (QC) function for in-depth analysis from raw data to results
With the new Quality Control (QC) analysis function in PEAKS® Studio 11, users can evaluate the statistics of raw data and/or results and gain insight into the properties of LC-MS acquisition. Designed for DDA and DIA data, this automated tool will provide elements for determining data quality and evaluating experimental settings.
7. Peptide Feature View
From data refinement to identification and quantification, the PEAKS 11 series is based on peptide features. Peptide features include a range of corresponding m/z values, retention time range, intensity of different molecular isoform formation, and ion mobility as a fourth dimension of information.
In the PEAKS Identification Results, users will find a “Features” tab that displays all the details of each peptide feature in the raw data in a visual and tabular way. In the Peptide Feature Table, PEAKS conveniently summarizes the details of features detected from LC-MS or LC-IMS/MS, including identified sequences determined by database search, DDA or de novo sequencing. Each feature is also associated with its corresponding protein view, spectrum view and LC-MS or LC-IMS/MS view for further inspection.
8. Protein View
The protein view enables protein analysis of complex biological samples in DDA and DIA workflows. For each protein, the sequence coverage view displays peptide maps with spectrum annotations for verification. Using PEAKS’s traditional de novo assisted database search, users can easily view the identified blue peptide sequences, while the gray bars indicate de novo tag matches only.
9. Peptide View
The peptide view provides a list of identified peptides by MS1 abundance. For each modified peptide, the confidence (Ascore) of the modification site is associated.
10. Quantification View
With the add-on module of PEAKS Q, PEAKS Studio can also simultaneously determine the relative protein abundance changes of a group of samples without prior knowledge of the proteins involved.