Thermo Scientific™ LipidSearch™ software LipidSearch 5.1 from Thermo Fisher Scientific

LipidSearch 5.1 cracked version is the leading automatic lipid identification software, providing unique algorithms and advanced technologies, designed to provide users with automation advantages, accurate and automatic identification of various complex data and biological samples, all data sets will be quickly and conveniently integrated into your report, and provide detailed sample group difference display, allowing users to better identify and improve workflows, providing a complete software database, complete and detailed sample types, everything will improve your work efficiency,

you can search the computer database of user-defined lipid species, “total component” MS level lipid identification. Then, based on the results obtained in the MS level identification, the MS/MS data is processed by searching for predicted mass spectrometry product ions to identify molecular lipid species.

The results of positive and negative ion MS or MS/MS experiments are then merged together to give a comprehensive and correct lipid annotation level by correlating individual lipid identifications. Download the new cracked version

Features
1. Automatic lipid identification
Unique peak detection algorithms are customized for MS/MS associated with LC data, including combining information from CID/HCD fragments and MS2/MS3 spectra using Thermo Fisher Scientific Orbitrap ID-X and Fusion Tribrid mass spectrometers. Product ion search and annotation alignment algorithms are optimized for non-targeted lipid identification.
2. Comprehensive database coverage
The LipidSearch software database contains more than two molecular lipid species (96 subclasses) and their predicted fragment ions, and provides the most complete coverage of different sample types, including cells, human plasma, insect larvae, plants, seed oils, tissues, and yeast.
3. Sample preparation
The lipid standards (Table 1) were obtained as powder or chloroform stock solutions from Avanti Polar Lipid (Alabaster, AL). A mixture of 21 different lipid standards was prepared by combining the stock solutions. The lipid mixture (500 μL) was mixed with 100 μL of SPLASHTM (Avanti 330707) labeled internal standard mixture in methanol and diluted with 4:2:1 isopropanol/methanol/chloroform and 20 mM ammonium formate to obtain an estimated final concentration range of 0.1~100 μg/mL.
4. Mass spectrometry
Mass spectrometry analysis was performed on an Orbitrap Fusion Lumos MS. The Orbitrape Fusion Lumos-MS was operated in full MS scan mode (resolution 120000 at m/z 200) followed by ddMS2 (120K resolution). The AGC target values ​​were set to 4E5 and 1E5 for MS and MS/MS scans, respectively. The maximum injection times were 50 ms and 450 ms for MS and MS/MS, respectively. HCD was performed with a step collision energy of 30 ± 10% for neg. 30 ± 5% in positive ion mode with an isolation window of 1.0 Da. The lipid mixture was injected at a flow rate of 3 μL/min. Additional experiments were performed on a Thermo Scientific™ Orbitrap Mass Spectrometer™ HF mass spectrometer operating at 240K res. for MS and 120K res. for MS/MS scanning 280.25–1000.90 Da with a step size of 1.0009 Da.
5. Data Analysis
Lipid identification was performed using LipidSearch 5.0 pre-release software. First, the MS data were searched for monoisotopic precursor ions specified in a user-editable database using the parameters given in Table 2. Next, product ion searches were performed using the parameters given in Table 3. Finally, the MS and MS/MS search results were merged into total composition (precursor) and molecular (product ion) results, respectively.

Key Benefits

Automated lipid identification Comprehensive database coverage
Unique peak detection algorithms tailored for MS/MS correlated with LC data include combining information from CID/HCD fragmentation and MS2/MS3 spectra using Thermo Fisher Scientific Orbitrap ID-X and Fusion Tribrid mass spectrometers. Product ion search and annotation alignment algorithms are optimized for non-targeted lipid identification.

The LipidSearch software database contains more than two dozen molecular lipid species (96 subclasses) and their predicted fragment ions and provides the most complete coverage of different sample types, including cells, human plasma, insect larvae, plants, seed oils, tissues, and yeast.

Thermo Scientific™ LipidSearch™ software LipidSearch 5.1

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, with annual revenue exceeding $30 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, improving patient diagnostics and therapies or increasing productivity in their laboratories, we are here to support them.

Our global team of more than 80,000 colleagues delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services and Patheon.

About Thermo Fisher Scientific LipidSearch 5.1

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, with annual revenue exceeding $30 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, improving patient diagnostics and therapies or increasing productivity in their laboratories, we are here to support them. Our global team of more than 80,000 colleagues delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services and Patheon. For more information, please visit www.thermofisher.com.

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Whether our customers are accelerating life sciences research, solving complex analytical challenges, or increasing productivity in their laboratories, we are here to support them.

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Thermo Fisher Scientific is the world leader in serving science and with that position comes a great sense of responsibility to the global community. We realize we have the ability to make a significant positive impact on society. The products and services we provide to our customers help them tackle some of the world’s greatest challenges. We also contribute by operating responsibly and by giving back to the communities in which we live and work.